janus automated liquid handling workstation (Revvity)
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Janus Automated Liquid Handling Workstation, supplied by Revvity, used in various techniques. Bioz Stars score: 94/100, based on 1682 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/janus+automated+liquid+handling+workstation/JANUS+liquid+handling+workstations/pmc13112130-160-50-55
Average 94 stars, based on 1682 article reviews
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Activity Assay:Article Title: Disulfiram potentiates cisplatin-induced apoptosis in small cell lung cancer via the inhibition of cystathionine β-synthase and H 2 S Article Snippet: Then, an equal volume of CellTiter-Glo reagent was added and incubated at room temperature for 10 min. Luminescent signal was measured in a multilabel plate reader (Molecular Devices, CA, USA). .. A Staining:Article Title: Cell Painting PLUS: expanding the multiplexing capacity of Cell Painting-based phenotypic profiling using iterative staining-elution cycles Article Snippet: .. The generation of the reference compound plate, preparation and application of exposure media to cells as well as all staining and elution procedures were performed using a Article Title: Cell Painting PLUS: expanding the multiplexing capacity of Cell Painting-based phenotypic profiling using iterative staining-elution cycles. Article Snippet: .. The generation of the reference compound plate, preparation and application of exposure media to cells as well as all staining and elution procedures were performed using a Cell Culture:Article Title: Cell Painting PLUS: An Iterative Staining‐Elution Protocol for High‐Content Phenotypic Screenings Article Snippet: 5 mg/ml collagen type 1, rat tail (Ibidi, cat. no. 50201) Dulbecco's Balanced Salt Solution (DPBS), without Ca and Mg (PAN Biotech, cat. no. P04‐36500), sterile Cell line, growing in a standard cell culture flask: U2OS (HTB‐96, ATCC); HepG2 (ACC 180, DSMZ), or MCF‐7/vBOS (Michigan Cancer Foundation‐7/variantBOS; Bischoff et al., ; Kornhuber, Dunst, Schönfelder, & Oelgeschläger, ) Cell dissociation solution: e.g., Accumax Cell Dissociation Solution (PAN Biotech, cat. no. P10‐21250) Cell growth medium appropriate for the cells used (U2OS, HepG2, or MCF‐7/vBOS; see recipes) 0.4% Trypan Blue stain (Invitrogen/Thermo Fisher Scientific, cat. no. T10282 ), for determination of cell viability Assay‐ready compound plate containing test compounds and control compounds (see Support Protocol ) 384‐well microplates for cell seeding, suitable for microscopy: e.g., PhenoPlates (Revvity, cat. no. 6057302) Sterile cell culture materials: e.g., cell culture flask (TPP, cat. nos. .. TPP90076 [T75] and TPP90151 [T150]) Cell culture incubator, 37°C, 5% CO 2 , and high humidity (∼95% relative humidity; e.g., Memmert ICO50) Serological pipets Conical tube Cell counting system: e.g., automated cell counter (Life Technologies/Thermo Fisher Scientific Countess II FL) Multichannel pipet Automated pipetting device suitable for 384‐well format: e.g., Cell Counting:Article Title: Cell Painting PLUS: An Iterative Staining‐Elution Protocol for High‐Content Phenotypic Screenings Article Snippet: 5 mg/ml collagen type 1, rat tail (Ibidi, cat. no. 50201) Dulbecco's Balanced Salt Solution (DPBS), without Ca and Mg (PAN Biotech, cat. no. P04‐36500), sterile Cell line, growing in a standard cell culture flask: U2OS (HTB‐96, ATCC); HepG2 (ACC 180, DSMZ), or MCF‐7/vBOS (Michigan Cancer Foundation‐7/variantBOS; Bischoff et al., ; Kornhuber, Dunst, Schönfelder, & Oelgeschläger, ) Cell dissociation solution: e.g., Accumax Cell Dissociation Solution (PAN Biotech, cat. no. P10‐21250) Cell growth medium appropriate for the cells used (U2OS, HepG2, or MCF‐7/vBOS; see recipes) 0.4% Trypan Blue stain (Invitrogen/Thermo Fisher Scientific, cat. no. T10282 ), for determination of cell viability Assay‐ready compound plate containing test compounds and control compounds (see Support Protocol ) 384‐well microplates for cell seeding, suitable for microscopy: e.g., PhenoPlates (Revvity, cat. no. 6057302) Sterile cell culture materials: e.g., cell culture flask (TPP, cat. nos. .. TPP90076 [T75] and TPP90151 [T150]) Cell culture incubator, 37°C, 5% CO 2 , and high humidity (∼95% relative humidity; e.g., Memmert ICO50) Serological pipets Conical tube Cell counting system: e.g., automated cell counter (Life Technologies/Thermo Fisher Scientific Countess II FL) Multichannel pipet Automated pipetting device suitable for 384‐well format: e.g., |
